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cell extraction buffer  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cell extraction buffer
    Cell Extraction Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+buffer/FastScan+Total+LC3B+ELISA+Kit/pm42721581-87-5-8
    Average 93 stars, based on 8 article reviews
    cell extraction buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate.
    Article Snippet: After washing the plate again for five times, 50 μL goatanti-mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA). .. Then, wells were washed five times with ELISA buffer, and subsequently incubated for 1 h at 37 °C with streptavidin-HRP antibody (1:1000 in 1:2 blocking buffer in ELISA buffer; Dako, Glostrup, Denmark). .. Finally, the plate was washed seven times with ELISA buffer, and incubated for 30 min at 37 °C with 100 μL TMB (Cell signaling, Danvers, MA, USA).

    Article Title: Feed compositions for preventing and treating inflammatory diseases
    Article Snippet: .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution. .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution.

    Article Title: Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2–1 and Prevents Doxorubicin-Induced Ileitis
    Article Snippet: Isolated TLR2 protein and HA peptide (Thermo Scientific, Waltham, MA, USA) were applied to pectin-coated wells at 0.33, 1, 3, and 9 μg/well; and incubated at 37°C for 3 h. HA peptide was used as a negative control. .. Afterward, wells were washed with 400 μl of ELISA buffer for five times and incubated with 50 μl primary HA tag antibody (Cell Signaling, Danvers, MA, USA) at 1:200 dilutions. .. Afterward, wells were washed with 400 μl of ELISA buffer for five times and incubated with 50 μl primary HA tag antibody (Cell Signaling, Danvers, MA, USA) at 1:200 dilutions.

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse‐anti‐HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After washing the plate again for five times, 50 μL goat‐anti‐mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA).

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: Then, wells were washed five times with ELISA buffer, and subsequently incubated for 1 h at 37 °C with streptavidin‐HRP antibody (1:1000 in 1:2 blocking buffer in ELISA buffer; Dako, Glostrup, Denmark). .. Finally, the plate was washed seven times with ELISA buffer, and incubated for 30 min at 37 °C with 100 μL TMB (Cell signaling, Danvers, MA, USA). .. The reaction was stopped by adding 100 μL stop solution (Cell signaling, Danvers, MA, USA).

    Article Title: Food and/or feed compositions for preventing and treating inflammatory diseases
    Article Snippet: .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution. .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution.

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate.
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse-anti-HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse-anti-HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA).

    Incubation:

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate.
    Article Snippet: After washing the plate again for five times, 50 μL goatanti-mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA). .. Then, wells were washed five times with ELISA buffer, and subsequently incubated for 1 h at 37 °C with streptavidin-HRP antibody (1:1000 in 1:2 blocking buffer in ELISA buffer; Dako, Glostrup, Denmark). .. Finally, the plate was washed seven times with ELISA buffer, and incubated for 30 min at 37 °C with 100 μL TMB (Cell signaling, Danvers, MA, USA).

    Article Title: Feed compositions for preventing and treating inflammatory diseases
    Article Snippet: .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution. .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution.

    Article Title: Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2–1 and Prevents Doxorubicin-Induced Ileitis
    Article Snippet: Isolated TLR2 protein and HA peptide (Thermo Scientific, Waltham, MA, USA) were applied to pectin-coated wells at 0.33, 1, 3, and 9 μg/well; and incubated at 37°C for 3 h. HA peptide was used as a negative control. .. Afterward, wells were washed with 400 μl of ELISA buffer for five times and incubated with 50 μl primary HA tag antibody (Cell Signaling, Danvers, MA, USA) at 1:200 dilutions. .. Afterward, wells were washed with 400 μl of ELISA buffer for five times and incubated with 50 μl primary HA tag antibody (Cell Signaling, Danvers, MA, USA) at 1:200 dilutions.

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse‐anti‐HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After washing the plate again for five times, 50 μL goat‐anti‐mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA).

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: Then, wells were washed five times with ELISA buffer, and subsequently incubated for 1 h at 37 °C with streptavidin‐HRP antibody (1:1000 in 1:2 blocking buffer in ELISA buffer; Dako, Glostrup, Denmark). .. Finally, the plate was washed seven times with ELISA buffer, and incubated for 30 min at 37 °C with 100 μL TMB (Cell signaling, Danvers, MA, USA). .. The reaction was stopped by adding 100 μL stop solution (Cell signaling, Danvers, MA, USA).

    Article Title: Food and/or feed compositions for preventing and treating inflammatory diseases
    Article Snippet: .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution. .. Afterwards, the wells were washed with 400 μl of ELISA buffer for 5 times and incubated with 50 μl primary detection antibody for the HA tag (Cell Signaling, Danvers, Mass., USA) in a 1:200 dilution.

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate.
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse-anti-HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse-anti-HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA).

    Blocking Assay:

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate.
    Article Snippet: After washing the plate again for five times, 50 μL goatanti-mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA). .. Then, wells were washed five times with ELISA buffer, and subsequently incubated for 1 h at 37 °C with streptavidin-HRP antibody (1:1000 in 1:2 blocking buffer in ELISA buffer; Dako, Glostrup, Denmark). .. Finally, the plate was washed seven times with ELISA buffer, and incubated for 30 min at 37 °C with 100 μL TMB (Cell signaling, Danvers, MA, USA).

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse‐anti‐HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After washing the plate again for five times, 50 μL goat‐anti‐mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA).

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate.
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse-anti-HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse-anti-HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA).

    other:

    Article Title: Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2-1 and Prevents Doxorubicin-Induced Ileitis.
    Article Snippet: ELISA plates (Corning, Tewksbury, MA, USA) were treated with 50 μl of 50 μg/ml of poly-l-lysine for 1 h at 37°C.

    Cell-Signaling:

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: .. After incubation, the plate was washed five times with ELISA buffer and incubated for 2 h at 37 °C with 50 μL mouse‐anti‐HA tag antibody (1:200 in 1:2 blocking buffer in ELISA buffer; Cell signaling, Danvers, MA, USA). .. After washing the plate again for five times, 50 μL goat‐anti‐mouse biotin antibody was applied to the wells for 1 h at 37 °C (1:500 in 1:2 blocking buffer in ELISA buffer; Southern Biotechnology, Birmingham, AL, USA).

    Article Title: Identification of a TLR2 Inhibiting Wheat Hydrolysate
    Article Snippet: Then, wells were washed five times with ELISA buffer, and subsequently incubated for 1 h at 37 °C with streptavidin‐HRP antibody (1:1000 in 1:2 blocking buffer in ELISA buffer; Dako, Glostrup, Denmark). .. Finally, the plate was washed seven times with ELISA buffer, and incubated for 30 min at 37 °C with 100 μL TMB (Cell signaling, Danvers, MA, USA). .. The reaction was stopped by adding 100 μL stop solution (Cell signaling, Danvers, MA, USA).



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